SCREENING OF PEPTIDE LIBRARIES LINKED TO LAC REPRESSOR

摘要: Publisher Summary Recently developed techniques allow the isolation of peptide ligands for any a variety receptor molecules. The most widely used technique constructing and screening very large libraries involves display on surface filamentous phage. Phage greater than 1010 different compounds can be screened efficiently, because phage particle makes connection between peptide, fused to coat protein outside virion, genetic material, encoding that contained within virion. This allows interest through binding properties peptides, then subsequent identification peptides ability material replicate yield sequence information. chapter describes protocols construction peptides-on-plasmids initial characterization resulting clones. are here have improved somewhat since publication original paper, describing technique. method is technically more demanding library screening. procedures described in paper those efficient enrichment receptor-specific peptides.