Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update.

摘要: The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents less than months. Despite rigorous global containment quarantine efforts limit the transmission virus, COVID-19 deaths have continued increase, leaving devastating impacts lives many with far-reaching effects society, economy healthcare system. With 43 million 1.1 recorded worldwide, accurate rapid diagnosis continues be cornerstone pandemic control. In this review, we aim present an objective overview latest nucleic acid-based diagnostic tests for detection SARS-CoV-2 that been authorized Food Drug Administration (FDA) under emergency use authorization (EUA) 31 October 2020. We systematically summarize compare principles, technologies, protocols performance characteristics amplification- sequencing-based become alternatives CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. highlight notable features including settings, along advantages disadvantages tests. conclude brief discussion current challenges future perspectives diagnostics.