COMBINATION OF FLOW CYTOMETRY AND SINGLE CELL ABSORPTION SPECTROSCOPY TO STUDY THE PHYTOPLANKTON STRUCTURE AND TO CALCULATE THE CHL A SPECIFIC ABSORPTION COEFFICIENTS AT THE TAXON LEVEL1
作者： Jörg Toepel , Uwe Langner , Christian Wilhelm
DOI: 10.1111/J.1529-8817.2005.00137.X
关键词: Biology 、 Analytical chemistry 、 Spectroscopy 、 Yield (chemistry) 、 Absorption (electromagnetic radiation) 、 Botany 、 Attenuation coefficient 、 Phytoplankton 、 PHY 、 Pigment 、 Absorption spectroscopy
摘要: The phytoplankton community structure of a hypertrophic lake was quantitatively determined with the aid flow cytometry. cytometry signals were calibrated to obtain cell-specific information, such as chl content and biovolume per cell. reliability this method tested laboratory cultures. results in respect distribution different algal groups obtained by compared from HPLC pigment fingerprinting. Both methods yield percentage contribution total a. specific absorption coefficient (a* Phy ) via visible (VIS) spectroscopy samples taken (Auensee) 2003. indicated that a* cell suspension is dependent on well environmental factors. linear relationship between at 675 nm product offered possibility normalize spectra acquire taxon-specific . estimated (675 nm) values used single wavelength 400 750 for representatives major groups. Our measurements show whole varies within season. Finally, we calculate light each group lake.
archives-ouvertes.fr
sci-hub.se 参考文章(56)
M. Gilbert, A. Domin, A. Becker, C. Wilhelm, Estimation of Primary Productivity by Chlorophyll a in vivo Fluorescence in Freshwater Phytoplankton Photosynthetica. ,vol. 38, pp. 111- 126 ,(2000) , 10.1023/A:1026708327185
S.W. Jeffrey, G.F. Humphrey, New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton Biochemie und Physiologie der Pflanzen. ,vol. 167, pp. 191- 194 ,(1975) , 10.1016/S0015-3796(17)30778-3
Jörg Toepel, Christian Wilhelm, Armin Meister, Annette Becker, Maria del Carmen Martinez-Ballesta, Cytometry of freshwater phytoplankton. Methods in Cell Biology. ,vol. 75, pp. 375- 407 ,(2004) , 10.1016/S0091-679X(04)75015-3
M. F. Wilkins, Lynne Boddy, C. W. Morris, R. R. Jonker, Identification of Phytoplankton from Flow Cytometry Data by Using Radial Basis Function Neural Networks Applied and Environmental Microbiology. ,vol. 65, pp. 4404- 4410 ,(1999) , 10.1128/AEM.65.10.4404-4410.1999
PA Staehr, P Henriksen, S Markager, Photoacclimation of four marine phytoplankton species to irradiance and nutrient availability Marine Ecology Progress Series. ,vol. 238, pp. 47- 59 ,(2002) , 10.3354/MEPS238047
ZOE V FINKEL, ANDREW J IRWIN, Modeling size-dependent photosynthesis: light absorption and the allometric rule. Journal of Theoretical Biology. ,vol. 204, pp. 361- 369 ,(2000) , 10.1006/JTBI.2000.2020
Rossella Barone, Luigi Naselli Flores, Phytoplankton dynamics in a shallow, hypertrophic reservoir (Lake Arancio, Sicily) Hydrobiologia. ,vol. 289, pp. 199- 214 ,(1994) , 10.1007/BF00007421
Michele D. DuRand, Rebecca E. Green, Heidi M. Sosik, Robert J. Olson, Diel Variations in Optical Properties of Micromonas pusilla, A prasinophyte Journal of Phycology. ,vol. 38, pp. 1132- 1142 ,(2002) , 10.1046/J.1529-8817.2002.02008.X
Rosângela A. Devilla, Murray T. Brown, Maria Donkin, James W. Readman, The effects of a PSII inhibitor on phytoplankton community structure as assessed by HPLC pigment analyses, microscopy and flow cytometry. Aquatic Toxicology. ,vol. 71, pp. 25- 38 ,(2005) , 10.1016/J.AQUATOX.2004.10.002
Terry L. Cucci, Michael E. Sieracki, Effects of mismatched refractive indices in aquatic flow cytometry. Cytometry. ,vol. 44, pp. 173- 178 ,(2001) , 10.1002/1097-0320(20010701)44:3<173::AID-CYTO1109>3.0.CO;2-5