A Mutation in the sap Operon Attenuates Survival of Nontypeable Haemophilus influenzae in a Chinchilla Model of Otitis Media

作者: Kevin M. Mason , Robert S. Munson , Lauren O. Bakaletz

DOI: 10.1128/IAI.73.1.599-608.2005

关键词: Beta defensinHaemophilus influenzaeOtitisVirologyMutantBiologyPasteurellaceaeMicrobiologyOperonPlasmidAntimicrobial peptides

摘要: Bacteria have evolved strategies to resist killing by antimicrobial peptides (APs), important effectors of innate immunity. The sap (sensitivity peptides) operon confers resistance AP-mediated Salmonella. We recently shown that sapA gene expression is upregulated in the middle ear a chinchilla model nontypeable Haemophilus influenzae (NTHI)-induced otitis media. Based on these findings, we constructed an NTHI strain containing Lux reporter plasmid driven promoter and demonstrated early yet transient within sites upper airway upon infection. hypothesized products mediate APs. In order test this hypothesis, nonpolar mutation 86-028NP, low-passage-number clinical isolate. mutant was approximately eightfold more sensitive than parent recombinant β-defensin 1. then assessed ability both colonize cause media chinchillas. significantly attenuated compared its survive nasopharynx chinchilla. addition, impaired compete with dual-strain challenge Our results indicate are for resisting activity APs may regulate, part, balance between normal carriage disease caused NTHI.

参考文章(64)
Gerard J. Barcak, Mark S. Chandler, Rosemary J. Redfield, Jean-Francois Tomb, Genetic systems in Haemophilus influenzae. Methods in Enzymology. ,vol. 204, pp. 321- 342 ,(1991) , 10.1016/0076-6879(91)04016-H
Victor Lorian, Antibiotics in laboratory medicine Published in <b>2005</b> in Philadelphia by Lippincott Williams & Wilkins. ,(2005)
M Perego, J A Hoch, Negative regulation of Bacillus subtilis sporulation by the spo0E gene product. Journal of Bacteriology. ,vol. 173, pp. 2514- 2520 ,(1991) , 10.1128/JB.173.8.2514-2520.1991
Ian D. Hiles, Maurice P. Gallagher, Derek J. Jamieson, Christopher F. Higgins, Molecular characterization of the oligopeptide permease of Salmonella typhimurium Journal of Molecular Biology. ,vol. 195, pp. 125- 142 ,(1987) , 10.1016/0022-2836(87)90332-9
Iwao Honjo, Haruo Takahashi, Masaharu Sudo, Ken Ishijima, Makito Tanabe, Pathophysiological and therapeutic considerations of otitis media with effusion from viewpoint of middle ear ventilation. International Journal of Pediatric Otorhinolaryngology. ,vol. 43, pp. 105- 113 ,(1998) , 10.1016/S0165-5876(97)00175-4
DJ Lim, YM Chun, HY Lee, SK Moon, KH Chang, J-D Li, A Andalibi, None, Cell biology of tubotympanum in relation to pathogenesis of otitis media — a review Vaccine. ,vol. 19, ,(2000) , 10.1016/S0264-410X(00)00273-5
Eduardo A. Groisman, How bacteria resist killing by host-defense peptides. Trends in Microbiology. ,vol. 2, pp. 444- 449 ,(1994) , 10.1016/0966-842X(94)90802-8
J SADE, A AR, Middle ear and auditory tube: Middle ear clearance, gas exchange, and pressure regulation Otolaryngology - Head and Neck Surgery. ,vol. 116, pp. 499- 524 ,(1997) , 10.1016/S0194-5998(97)70302-4
Lin Guo, Kheng B Lim, Cristina M Poduje, Morad Daniel, John S Gunn, Murray Hackett, Samuel I Miller, Lipid A Acylation and Bacterial Resistance against Vertebrate Antimicrobial Peptides Cell. ,vol. 95, pp. 189- 198 ,(1998) , 10.1016/S0092-8674(00)81750-X