Antigen-Antibody Binding and Mass Transport by Convection and Diffusion to a Surface: A Two-Dimensional Computer Model of Binding and Dissociation Kinetics

摘要: Abstract The kinetics of binding and dissociation between a soluble analyte an immobilized ligand on or near surface are described numerically by iterative computer model. model is applied to microflow chamber which used for plasmon resonance measurements. It calculates diffusion perpendicular the surface, flow parallel interaction any number species. If reaction fast, influenced transport away from in this case measurement yields apparent association rate constants not identical with ligand. transition mass transport-controlled processes reaction-controlled attention drawn possible misinterpretations experimental curves. higher than allowed conventional technique can be performed elution second low molecular weight.The weight.