Mechanisms of olfactory ensheathing cell-enhanced neurite outgrowth and axon regeneration after spinal cord injury

摘要: Olfactory ensheathing cells (OECs) provide a pro-regenerative environment for the axons of olfactory receptor neurons and therefore are promising candidate cell transplantation therapy following spinal cord injury. We previously showed that OEC supports axon regeneration functional re-connectivity complete injury, yet lack an OEC-specific marker limited our ability to determine how they promoted these beneficial effects. Using both in vitro vivo models, we investigated mechanisms by which OECs mediate regeneration. enhance neurite outgrowth postnatal cortical scar-like culture model. strong evidence direct OEC-neurite alignment is critical astrocyte meningeal fibroblast inhibitory environments. also tested eGFP-OECs from transgenic rats facilitate vitro. Then short-term study, analyzed survival, migration, distribution within lesion site transected rats. found transplanted with preserve associate core, reduce presence CSPGs myelin debris, secondary tissue damage due microglial macrophage activation infiltration post-injury. Collectively, data support neuroprotective proregenerative role through modulation glial scar formation transection.