Intracellular Chloride Regulation in AVP+ and VIP+ Neurons of the Suprachiasmatic Nucleus
作者： Nathan J. Klett , Charles N. Allen
DOI: 10.1038/S41598-017-09778-X
关键词: Transporter 、 Endocrinology 、 Suprachiasmatic nucleus 、 Bumetanide 、 Internal medicine 、 Excitatory postsynaptic potential 、 Receptor 、 Chemistry 、 Circadian rhythm 、 Intracellular 、 Inhibitory postsynaptic potential 、 Biophysics
摘要: Several reports have described excitatory GABA transmission in the suprachiasmatic nucleus (SCN), master pacemaker of circadian physiology. However, there is disagreement regarding prevalence, timing, and neuronal location SCN. Whether inhibitory or depends, part, on intracellular concentration chloride ([Cl−]i). Here, using ratiometric Cl− imaging, we investigated regulation AVP VIP-expressing SCN neurons found evidence suggesting that [Cl−]i higher during day than night both AVP+ VIP+ neurons. We then contribution cation cotransporters to setting these uptake transporter NKCC1 contributes neurons, but KCCs are primary regulators Interestingly, observed differentially regulated between neurons-a low loop diuretic bumetanide had differential effects while blocking with VU0240551 a larger effect compared
nature.com
elsevier.com
harvard.edu
sci-hub.se 参考文章(72)
Yehezkel Ben-Ari, Melanie A. Woodin, Evelyne Sernagor, Laura Cancedda, Laurent Vinay, Claudio Rivera, Pascal Legendre, Heiko J. Luhmann, Angelique Bordey, Peter Wenner, Atsuo Fukuda, Anthony N. van den Pol, Jean-Luc Gaiarsa, Enrico Cherubini, Refuting the challenges of the developmental shift of polarity of GABA actions: GABA more exciting than ever! Frontiers in Cellular Neuroscience. ,vol. 6, pp. 35- 35 ,(2012) , 10.3389/FNCEL.2012.00035
M. D. C. Belle, C. O. Diekman, D. B. Forger, H. D. Piggins, Daily electrical silencing in the mammalian circadian clock. Science. ,vol. 326, pp. 281- 284 ,(2009) , 10.1126/SCIENCE.1169657
John M. Russell, Sodium-Potassium-Chloride Cotransport Physiological Reviews. ,vol. 80, pp. 211- 276 ,(2000) , 10.1152/PHYSREV.2000.80.1.211
Peter Blaesse, Matti S. Airaksinen, Claudio Rivera, Kai Kaila, Cation-chloride cotransporters and neuronal function. Neuron. ,vol. 61, pp. 820- 838 ,(2009) , 10.1016/J.NEURON.2009.03.003
Robert P. Irwin, Charles N. Allen, GABAergic signaling induces divergent neuronal Ca2+ responses in the suprachiasmatic nucleus network European Journal of Neuroscience. ,vol. 30, pp. 1462- 1475 ,(2009) , 10.1111/J.1460-9568.2009.06944.X
Robert Mason, Stephany M. Biello, Mary E. Harrington, The effects of GABA and benzodiazepines on neurones in the suprachiasmatic nucleus (SCN) of Syrian hamsters. Brain Research. ,vol. 552, pp. 53- 57 ,(1991) , 10.1016/0006-8993(91)90659-J
Liisa A. Tremere, Raphael Pinaud, Robert P. Irwin, Charles N. Allen, Postinhibitory rebound spikes are modulated by the history of membrane hyperpolarization in the SCN European Journal of Neuroscience. ,vol. 28, pp. 1127- 1135 ,(2008) , 10.1111/J.1460-9568.2008.06410.X
E. Delpire, E. Days, L. M. Lewis, D. Mi, K. Kim, C. W. Lindsley, C. D. Weaver, Small-molecule screen identifies inhibitors of the neuronal K-Cl cotransporter KCC2 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 106, pp. 5383- 5388 ,(2009) , 10.1073/PNAS.0812756106
Kevin Strange, Thomas D. Singer, Rebecca Morrison, Eric Delpire, Dependence of KCC2 K-Cl cotransporter activity on a conserved carboxy terminus tyrosine residue American Journal of Physiology-cell Physiology. ,vol. 279, ,(2000) , 10.1152/AJPCELL.2000.279.3.C860
Nicolas Doyon, Laurent Vinay, Steven A. Prescott, Yves De Koninck, Chloride Regulation: A Dynamic Equilibrium Crucial for Synaptic Inhibition. Neuron. ,vol. 89, pp. 1157- 1172 ,(2016) , 10.1016/J.NEURON.2016.02.030