Staphylococcal enterotoxin a detection with phage displayed antibodies
作者： K. K. Fursova , M. P. Shchannikova , A. O. Shepelyakovskaya , L. L. Pavlik , F. A. Brovko
DOI: 10.1134/S1068162016020035
关键词: Antibody 、 Immunoassay 、 Staphylococcal enterotoxin A 、 Molecular biology 、 Monoclonal 、 Arginine 、 Trehalose 、 Detection limit 、 Microbiology 、 Chemistry 、 Functional activity
摘要: A technique for detection of staphylococcal enterotoxin with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture and phage displayed scFv antibodies. The limit 6–12.5 ng/mL different pairs Some conditions phage-displayed storage in dissolved lyophilized state examined. It shown the use trehalose arginine preserved functional activity
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