Comparison of changes in toxicity during growth of Anabaena circinalis (cyanobacteria) determined by mouse neuroblastoma bioassay and HPLC
作者： Vincent Testé , Jean-François Briand , Brenton C. Nicholson , Simone Puiseux-Dao
关键词: Cyanobacteria 、 Toxicity 、 Biological activity 、 Bioassay 、 Microbiology 、 Anabaena circinalis 、 Paralytic shellfish poisoning 、 Chromatography 、 Toxin 、 Saxitoxin 、 Biology
摘要: Changes in toxicity were studied during batch culture of a strain ofAnabaena circinalis (AWQC ANA-311F) producing paralyticshellfish toxins (PSTs). PSTs extracted from cells and mediumduring the period 29 days. Samples analysed by HPLC withpost-column oxidation coupled to fluorescence detection, tested with themouse neuroblastoma bioassay (MNB). Seven detected, but only fivewerequantified. Based on chemical analysis HPLC, a‘theoretical’ saxitoxin concentration related mouse wascalculated. Neuroblastoma cell survival was measured MNB andcorrelated total A. samples.Comparison made between changes shown the‘theoretical’ HPLC. There asignificant positive correlation two medium, notthe cells.
springer.com
sci-hub.se 参考文章(26)
Yasukatsu Oshima, Postcolumn derivatization liquid chromatographic method for paralytic shellfish toxins Journal of AOAC International. ,vol. 78, pp. 528- 532 ,(1995) , 10.1093/JAOAC/78.2.528
Andrew P Negri, Gary J. Jones, Susan I. Blackburn, Yasukatsu Oshima, Hideyuki Onodera, Effect of culture and bloom development and of sample storage on paralytic shellfish poisons in the cyanobacterium Anabaena circinalis Journal of Phycology. ,vol. 33, pp. 26- 35 ,(1997) , 10.1111/J.0022-3646.1997.00026.X
Marie-Yasmine Dechraoui, Jérôme Naar, Serge Pauillac, Anne-Marie Legrand, Ciguatoxins and brevetoxins, neurotoxic polyether compounds active on sodium channels Toxicon. ,vol. 37, pp. 125- 143 ,(1999) , 10.1016/S0041-0101(98)00169-X
Paulo Pereira, Hideyuki Onodera, Darı́o Andrinolo, Susana Franca, Filomena Araújo, Néstor Lagos, Yasukatsu Oshima, Paralytic shellfish toxins in the freshwater cyanobacterium Aphanizomenon flos-aquae, isolated from Montargil reservoir, Portugal Toxicon. ,vol. 38, pp. 1689- 1702 ,(2000) , 10.1016/S0041-0101(00)00100-8
Francesco Pomati, Silvia Sacchi, Carlo Rossetti, Stefano Giovannardi, Hideyuki Onodera, Yasukatsu Oshima, Brett A. Neilan, THE FRESHWATER CYANOBACTERIUM PLANKTOTHRIX SP. FP1: MOLECULAR IDENTIFICATION AND DETECTION OF PARALYTIC SHELLFISH POISONING TOXINS Journal of Phycology. ,vol. 36, pp. 553- 562 ,(2000) , 10.1046/J.1529-8817.2000.99181.X
Gary J. Jones, Andrew P. Negri, Persistence and degradation of cyanobacterial paralytic shellfish poisons (PSPs) in freshwaters Water Research. ,vol. 31, pp. 525- 533 ,(1997) , 10.1016/S0043-1354(96)00134-0
Miyoshi Ikawa, Karin Wegener, Thomas L. Foxall, John J. Sasner, Comparison of the toxins of the blue-green alga Aphanizomenon flos-aquae with the Gonyaulax toxins Toxicon. ,vol. 20, pp. 747- 752 ,(1982) , 10.1016/0041-0101(82)90122-2
Nik A. Mahmood, Wayne W. Carmichael, Paralytic shellfish poisons produced by the freshwater cyanobacterium Aphanizomenon flos-aquae NH-5. Toxicon. ,vol. 24, pp. 175- 186 ,(1986) , 10.1016/0041-0101(86)90120-0
Bertil Hille, Ionic channels of excitable membranes ,(1984)
Joanne F. Jellett, Linda J. Marks, James E. Stewart, Maria L. Dorey, Wendy Watson-Wright, James F. Lawrence, Paralytic shellfish poison (saxitoxin family) bioassays: automated endpoint determination and standardization of the in vitro tissue culture bioassay, and comparison with the standard mouse bioassay. Toxicon. ,vol. 30, pp. 1143- 1156 ,(1992) , 10.1016/0041-0101(92)90430-D