Poly (ethylene-co-vinyl alcohol) is a suitable substrate for human olfactory neuroepithelial cell differentiation in vitro through a defined regulatory pathway.

摘要: Abstract Olfactory dysfunction significantly influences patients’ life quality, but currently has no adequate treatment. Poly (ethylene-co-vinyl alcohol) (EVAL) mediates cell adhesion, growth and modulates differentiation of neural stem cells. However, whether EVAL is a suitable substrate to establish an in vitro culture system that can promote development human olfactory neuroepithelial cells (HONCs) remains unexplored. This study isolates cultures HONCs on controls films for 21 days. The effects treatment are assessed using immunocytochemistry, microarray analysis, quantitative PCR, ELISA western blots following culturing. Most the morphology epithelial expresses markers sustentacular (SCs), cadherin-1 cytokeratin18, whereas main population presents as with extended thin processes possesses mature sensory neurons (OSNs), marker protein (OMP). Microarray analyses reveal neuropeptide Y (NPY) amphiregulin (AREG) two important regulating factors films. cultured enhance OSNs through NPY signaling, decrease SCs by blocking epidermal factor receptor (EGFR) activation. potential biomaterial serve ideal treating future. Statement Significance Olfaction not only contributes enjoyments food, provides clue escape from dangerous environmental hazards. loss smell commonly progressive there good prognostic approach dysfunction. Here, we use poly promotes We show enhances neuronpeptide protects neuroepithelium metaplasia inhibiting EGFR Therefore,