A long-term culture system for the expansion of hematopoietic stem cells from embryonic yolk sac with the capacity to seed erythroid and lymphoid development in vitro and to reconstitute the lymphoid compartment in severe combined immunodeficient mice.

摘要: : We have established a long-term culture system under which hematopoietic stem cells derived from the embryonic yolk sac may be maintained for long periods. Evidence persistence of in this is provided by three experimental observations. First, erythroid progenitors, as evidenced morphological, functional, and phenotypical analysis, generated these cultures after more than 7 months culture. The progenitors are distinct those bone marrow both qualitatively quantitatively. This high colony plating efficiency, large size, different growth factor requirements, increased sensitivity to Epo other cytokines, well significant prolonged expansion capability, suggesting that proliferative primitive their analogs. Second, conditions, lymphoid also term presence appropriate cytokines. Third, preliminary data suggest engraftment reconstitution at least some compartments host animals. will provide useful model study early hematopoiesis, potential serving donor cell transplantation.