Awake 18F-FDG PET imaging of memantine-induced brain activation and test-retest in freely running mice

摘要: PET scans of the mouse brain are usually performed with anesthesia to immobilize animal. However, it is desirable avoid confounding factor in mouse-brain response. Methods: We developed and validated imaging awake, freely moving mice. Head-motion tracking was using radioactive point-source markers, we used information for PET-image motion correction. Regional 18F-FDG uptake a test, retest, memantine-challenge study measured awake (n = 8) anesthetized C57BL/6 An period considered scans. Results: Awake (motion-corrected) images showed an pattern comparable The test-retest variability (represented by intraclass correlation coefficient) regional SUV quantification animals (0.424-0.555) marginally lower than that (intraclass coefficient, 0.491-0.629) over different regions. increased memantine-induced more pronounced (+63.6%) (+24.2%) animals. Additional behavioral information, acquired animals, motor activity on memantine challenge (total distance traveled, 18.2 ± 5.28 m) compared (6.49 2.21 m). Conclusion: present method enables mice, thereby avoiding effects reading. It allows simultaneous measurement during acquisitions. does not require any additional hardware, can be deployed typical high-throughput scan protocols.