Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type.

摘要: Abstract There have been few studies focused on the molecular characterization of invasive Staphylococcus aureus strains in patients with diabetes Iran. In present study, 20 S. recovered from characterized by virulence and resistance analysis, biofilm formation, staphylocoagulase (SC) typing, protein A locus (spa) typing staphylococcal cassette chromosome mec (SCCmec) multilocus sequence (MLST). Virulence gene detection indicated a high prevalence encoding pvl genes (50%), low tst seg (each them was 5%) markedly fnbB (95%), fnbA (85%), icaD (75%), icaA (65%). total 3 coagulase types (III, 85%; II, 10%; V, 5%), 2 agr (I, 90%; II 10%) SCCmec (IV, 65%; III, 35%) four different clones namely ST8-MRSA-IV/t008 (50%) (USA300), ST239-MRSA-III/t030 (35%), ST5-MRSA-IV/t002 (10%), ST45-MRSA-IV/t038 (5%) were detected this study. Eighty-five percent isolates producers. All 4 high-level mupirocin (HLMUPR) belonged to CC/ST8-MRSA-IV/t008 clone carried mupA gene. Fusidic acid-resistant isolate ST239-SCCmec III/t030 clone. One vancomycin-intermediate our which ST5-MRSA-IVt002. Circulating MRSA (USA300) isolated highlighting possibility transmission these microorganisms' between hospital, community, environments. However, further require providing critical insights into importance continued controlling treatment infections diabetes.