Paroxysmal Permeability Disorders: Development of a Microfluidic Device to Assess Endothelial Barrier Function.

摘要: Background: Paroxysmal Permeability Disorders (PPDs) are pathological conditions caused by periodic short lasting increase of endothelial permeability, in the absence inflammatory, degenerative, ischemic vascular injury. PPDs include primary angioedema, idiopathic systemic capillary leak syndrome and some rare forms localized retroperitoneal-mediastinal edema. Aim: to validate a microfluidic device study permeability flow conditions. Materials Methods: we designed microchannel network (the smallest channel is 30μm square section). Human Umbilical Vein Endothelial Cells (HUVECs) were cultured under constant shear stress networks. assessment was based on interaction biotinylated fibronectin used as matrix for HUVECs FITC-conjugated avidin. The identified changes fluorescence intensity detected confocal fluorescent microscopy. Results: microchannels constantly perfused with steady culture medium, ensuring physiologically relevant level at wall ~0.2 Pa. Our preliminary results demonstrated that circulation medium or plasma from healthy volunteers associated low matrix. When bradykinin diluted perfused, an average detected. Conclusion: microvasculature model suitable functions physiological presence factors like known mediator several PPDs. Therefore, it can be promising tool better understand mechanisms underlying disorders permeability.