Transcription of nucleosomes from human chromatin

摘要: Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA nucleosomes is accessible DNA-dependent RNA polymerase vitro. The template capacities chromatin and 1.5 10%, respectively, relative high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes transcripts, 150, 120 95 nucleotides length, obtained when used templates. However, nucleosomal a template, predominant size transcripts 150 nucleotides. When oligonucleosomes templates longer obtained. This indicates can transcribe contained nucleosomes.