Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors.

摘要: To assess the differences in proteolytic activity of acute and chronic wound environments, fluids were collected from surgical wounds (22 samples) (25 various etiologies, including mixed vessel disease ulcers, decubiti diabetic foot ulcers. Matrix metalloproteinase (MMP) measured using Azocoll assay was significantly elevated by 30 fold (median 22.8 microg MMP Eq/ml) compared to 0.76 (p < 0.001). The addition matrix inhibitor Illomostat decreased approximately 90% all samples, confirming that majority due metalloproteinases. Gelatin zymograms indicated predominantly metalloproteinase-9 with smaller elevations metalloproteinase-2. In tissue metalloproteinase-1 levels analyzed a small subset wounds. When protease there an inverse correlation = 0.02, r - 0.78). vitro degradation epidermal growth factor 125I labelled fluid samples. There higher samples (mean 28.1%) 0.6%). This also correlated these 0. 001, 0.64). Additionally, proteases assayed 15 venous leg ulcers during nonhealing healing phase unique model humans. Patients admitted hospital for bed rest on admission (nonhealing phase) after 2 weeks (healing when had begun heal as evidenced reduction size 12%). These data showed occurs 0.01). parallels processes observed normally supports case inhibitors conjunction any treatments factors.