Tex19, a mammalian-specific protein with a restricted expression in pluripotent stem cells and germ line.

摘要: Although the properties of embryonic stem (ES) cells make these very attractive in field replacement therapy, molecular mechanisms involved maintenance their pluripotency are not fully characterized. Starting from observation that most pluripotent markers also expressed by spermatogonia cells, we identified Tex19 as a new potential marker. We show is mammalian-specific protein duplicated mouse and rat, renamed Tex19.1 Tex19.2, whereas only one form found human. In mouse, both forms localized on chromosome 11 transcribed opposite directions. proteins well conserved, showing two highly conserved domains do present any similarity with other known domains. Tex19.2 specifically detected male somatic gonad lineage, expression similar to Oct4. Transcripts maternally inherited, starts soon early embryo later limited germ line. transcripts were Tex19.1, like Oct4, decreases after murine cell differentiation. Human TEX19 was more closely related adult testis undifferentiated ES cells. By immunofluorescence, localizes nucleus inner mass All results suggest human TEX19, could be factor self-renewal or cells. Disclosure conflicts interest at end this article.