Uptake of endogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae

摘要: Sphingolipids are abundant and essential molecules in eukaryotes that have crucial functions as signaling membrane components. Sphingolipid biosynthesis starts the endoplasmic reticulum with condensation of serine palmitoyl-CoA. is highly regulated to maintain sphingolipid homeostasis. Even though, an component pathway, its role maintaining homeostasis has not been precisely studied. Here we show uptake important factor for regulation Saccharomyces cerevisiae . Using genetic experiments, find broad-specificity amino acid permease Gnp1 be uptake. We confirm these results assays gnp1 D cells. further exogenous by cellular levels observe a specific connection between first step biosynthesis. mass spectrometry-based flux analysis, observed imported main source de novo Our demonstrate yeast cells preferentially use regulate study can also starting point analyze mammalian metabolism.