Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.
作者： Chao Wu , Xueying Liu , Yufei Li , Xiaoyu Du , Xia Wang
DOI: 10.1016/J.BIOS.2013.09.040
关键词: Biosensor 、 Lipase 、 Detection limit 、 Nanoporous 、 Chemistry 、 Tributyrin 、 Biocomposite 、 Nuclear chemistry 、 Organic chemistry 、 Immobilized enzyme 、 Cyclic voltammetry
摘要: For triglycerides biosensor design, protein immobilization is necessary to create the interface between enzyme and electrode. In this study, a glassy carbon electrode (GCE) was modified with lipase-nanoporous gold (NPG) biocomposite (denoted as lipase/NPG/GCE). Due highly conductive, porous, biocompatible three-dimensional structure, NPG suitable for immobilization. cyclic voltammetry experiments, lipase/NPG/GCE bioelectrode displayed surface-confined reaction in phosphate buffer solution. Linear responses were obtained tributyrin concentrations ranging from 50 250 mg dl(-1) olive oil 10 200 dl(-1). The value of apparent Michaelis-Menten constant 10.67 detection limit 2.68 Further, had strong anti-interference ability against urea, glucose, cholesterol, uric acid well long shelf-life. human serum, values given by good agreement those an automatic biochemical analyzer. These properties along self-life make excellent choice construction biosensor.
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