Anti-JL1 antibody-conjugated poly (l-lysine) for targeted gene delivery to leukemia T cells

摘要: Abstract We have designed the gene delivery carrier targeted to Molt 4 cells, human leukemia T using monoclonal antibody against leukemia-specific JL1 antigen, anti-JL1 antibody, as a targeting moiety. Anti-JL1 has been proven bind antigen and subsequently be internalized into demonstrating that potential ligand for transfer. was modified with heterobifunctional crosslinker, PDPH, at carbohydrate sites conjugated thiolated poly- l -lysine (PLL) via disulfide bridges. The composition binding affinity of antibody–PLL conjugates were analyzed by amino acid analysis flow cytometry, respectively. Antibody–PLL neutralized pSV-β-galactosidase plasmid DNA 5:1 weight ratio condensed about 200–300-nm complexes. DNA/antibody–PLL complexes effectively cells after h incubation 37°C showed significantly higher in vitro transfection efficiency than DNA/PLL DNA/Lipofectin™ formulation due effect receptor-mediated endocytosis induced antibody.