A Versatile tRNA Aminoacylation Catalyst Based on RNA

摘要: Abstract Aminoacyl-tRNA synthetase (ARS) ribozymes have potential to develop a novel genetic coding system. Although we previously isolated such ribozyme that recognizes aromatic amino acids, it could not be used as versatile catalyst due its limited ability of aminoacylation particular tRNA for the selection. To overcome this limitation, combination evolutionary and engineering approaches generate an optimized ribozyme. The ribozyme, consisting 45 nucleotides, displays broad spectrum activity toward various tRNAs. Most significantly, is able exhibit multiple turnover charge parasubstituted Phe analogs onto engineered suppressor (tRNA Asn CCCG ). Thus, provides useful flexible tool custom synthesis mischarged tRNAs with natural nonnatural acids.