Development and characterization of 30 microsatellite loci for Plagiorhegma dubium (Berberidaceae)
作者： Bo-Yun Kim , Soo-Rang Lee , Yong-In Kim , Dae-Hyun Kang , Young-Dong Kim
DOI: 10.1002/APS3.1200
关键词: Berberidaceae 、 Endangered species 、 Loss of heterozygosity 、 Allele 、 Genetic diversity 、 Evolutionary biology 、 Population 、 Plagiorhegma dubium 、 Microsatellite 、 Biology
摘要: Premise of the Study Plagiorhegma dubium (Berberidaceae) has been listed as an endangered species in Korea due to extensive collection and destruction natural habitats. In this study, 30 microsatellite loci, including 25 polymorphic were developed for P. use population-level genetic analyses. Methods Results We carried out transcriptome sequencing isolated a total expressed sequence tag-simple repeat markers from using Illumina HiSeq high-throughput sequencing. To test utility markers, we genotyped 60 individuals three populations estimated number alleles levels observed expected heterozygosity. Expected heterozygosity ranged 0.000 0.594, 1.000, 0.744 populations, respectively. Conclusions These transcriptome-derived simple are highly can be widely used characterization dubium. Population studies with these will provide valuable insights conservation by unraveling evolutionary patterns
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