Metabolism of acetylene by Nocardia rhodochrous.

摘要: A Nocardia rhodochrous strain capable of utilizing acetylene as its sole source carbon and energy exhibited slow growth on low concentrations acetaldehyde. Resting cells incubated with formed a product identified acetaldehyde, but attempts to demonstrate hydrase activity in cell-free extracts were unsuccessful. Acetaldehyde dehydrogenase N. was found be NAD+ linked nonacylating, converting acetaldehyde acetate. Specific activities dehydrogenase, acetothiokinase, isocitrate lyase enhanced grown ethanol compared alternate substrates. These results suggest that is catabolized via acetate eventually acetyl coenzyme A. Acetylene oxidation appears constitutive not inhibited the presence either ethylene, nitrous oxide, or methane.