Method of determining the three-dimensional shape of a macromolecule

摘要: The present invention provides a fast and efficient method for determining the three-dimensional conformation of protein. steps include: 1) formation physical distance constraints, e.g., forming intramolecular chemical crosslinks known size between residues protein; 2) enriching number molecules that have in reaction pool, using separation to remove proteins with intermolecular bonds; 3) exposing enriched pool protease cuts protein at specific sites produce peptide fragments; 4) measuring fragments determine linkage certain spatial relationship 5) interpreting data produced geometry structure based on deduced sites. information is preferably analyzed aid from computer system, which can be used generate and/or analyze constraints amino acids.