Retention of paclitaxel in cancer cells for 1 week in vivo and in vitro

摘要: Purpose: Clinically, the administration of paclitaxel for ovarian cancer on a dose-dense weekly schedule, rather than conventional every-3-week might demonstrate greater tumor-cell death. Here, we investigate pharmacokinetics and pharmacodynamics in cells vivo vitro. Experimental design: Paclitaxel concentrations were measured by HPLC, apoptotic detected TUNEL assay paclitaxel-pretreated cervical treated with (10 ng/ml) tissues patients (60 mg/m2/week). Polymerized tubulin was polymerization assay, BrdU cell proliferation used to assess effect paclitaxel. Results: remained six 6 days after last medication. In vitro, retained all lines 24 h its removal from medium, still detectable CaSki day 7. Simultaneous treatment depolymerizing drugs inhibited retention paclitaxel-induced tubulin. After treatment, 1 week. Under high magnification, day 7 showed multinucleation. Conclusions: is unusual that it accumulates especially induces apoptosis 1 week On other hand, could not be 2 weeks. The standard produce