Photochemical cross-linking analysis of protein-nucleic acid interactions in Escherichia coli transcription complexes from lambda PR' promoter.

摘要: Publisher Summary This chapter describes the approaches used to characterize transcription complex that forms on bacteriophage λ (A) P R promoter, which is regulated by transcriptional antitermination. synthesizes viral proteins N and Q, reprogram host RNA polymerase allow expression of genes. Antitermination A Q protein differs from involving in several ways. First, a DNA-binding its site action inseparable promoter. interacts with promoter DNA about -31 -10, thus binds at overlapping upstream start site. Polymerase initiates pauses after incorporation first 15 or 16 nucleotides, where it modified NusA an antitermination form. It shown nontranscribed strand important for pause +16. Whether modifies then left behind, whether carried along modification, as case N, not known. Similarly, any portion nascent remains also