Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA.

摘要: F107 fimbriae were isolated and purified from edema disease strain 107/86 of Escherichia coli. Plasmid pIH120 was constructed, which contains the gene cluster that codes for adhesive fimbriae. The major fimbrial subunit gene, fedA, sequenced. An open reading frame a protein with 170 amino acids, including 21-amino-acid signal peptide, found. without sequence has calculated molecular mass 15,099 Da. Construction nonsense mutation in fedA abolished both expression capacity to adhere porcine intestinal villi. In screening 28 reference strains isolates clinically ill piglets, detected 24 cases (85.7%). 20 (83.3%) these strains, found association Shiga-like toxin II variant genes, coding is characteristic E. not enterotoxigenic coli strains. Because HB101(pIH120) transformants villi, high prevalence correlation toxin-encoding we suggest are an important virulence factor