Influence of carrier protein conjugation site and terminal modification of a GnRH-I peptide sequence in the development of a highly specific anti-fertility vaccine part I

摘要: PROBLEM: We previously immunoneutralized gonadotrophin releasing hormone (GnRH), using an analogue of GnRH (des-1 GnRH-1), conjugated to tetanus toxoid via a carbodiimide reaction. The castration effect on the reproductive system was not consistent in all treated animals. Therefore, we examined possibility that conjugation carrier protein N- or C-terminal could have efficacy. METHOD OF STUDY: sequences were synthesized consisting additional cysteine at either terminal and specific carried out bifunctional linker agent. RESULTS: Conjugation monomer through N-terminal proved be highly effective means causing immunocastration terms decreased testosterone concentrations testicular size, whereas ineffective. This reflected ability antibodies bind native GnRH, but levels anti-GnRH antibodies. CONCLUSION: Immunoneutralization efficacy attributed importance preserving C-terminal.