Extracellular production of an intact and biologically active human growth hormone by the Bacillus brevis system

摘要: The characteristic features of the Bacillus brevis system are very high productivity heterologous proteins and low extracellular protease activity. However, degradation some proteins, especially mammalian can be observed resulted in a lowering protein productivity. By using mutant expressing levels proteases addition EDTA to medium, intact human growth hormone (hGH) was successfully produced with B. system. Signal peptide modification higher basicity amino terminal region hydrophobicity middle brought about twelve-fold increase hGH production. yield further elevated 240 mg L-1 by optimization culture conditions. Thus, biologically active mature efficiently directly medium