c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
作者： Yaara Oppenheimer‐Shaanan , Ezequiel Wexselblatt , Jehoshua Katzhendler , Eylon Yavin , Sigal Ben‐Yehuda
关键词: Bacillus subtilis 、 Enzyme activator 、 G2-M DNA damage checkpoint 、 Enzyme 、 DNA damage 、 Sporulation in Bacillus subtilis 、 DNA 、 Biochemistry 、 Second messenger system 、 Biology
摘要: The bacterium Bacillus subtilis produces the DNA integrity scanning protein (DisA), a checkpoint that delays sporulation in response to damage. DisA scans chromosome and pauses at sites of lesions. Structural analysis showed synthesizes small molecule cyclic diadenosine monophosphate (c-di-AMP). Here, we demonstrate intracellular concentration c-di-AMP rises markedly onset DisA-dependent manner. Furthermore, exposing sporulating cells DNA-damaging agents leads global decrease level this molecule. This drop was associated with stalled complexes halt production increased levels c-di-AMP-degrading enzyme YybT. Reduced cause delay can be reversed by external supplementation Thus, acts as secondary messenger, coupling progression sporulation.
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