Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography.

作者: B L Levinson , K J Kasyan , S S Chiu , T C Currier , J M González

DOI: 10.1128/JB.172.6.3172-3179.1990

关键词: BacillalesColorado potato beetleSerotypeMicrobiologyExotoxinBacillus thuringiensisPlasmidToxinBiologyBacillaceae

摘要: An improved high-performance liquid chromatography separation was developed to detect and quantify beta-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin assigned a plasmid five strains, from three subspecies (B. subsp. serotype 1, B. tolworthi 9, darmstadiensis 10). A new exotoxin, called type II this report, discovered morrisoni 8ab, purified, partially characterized. This material is more specific than I very active against the Colorado potato beetle, Leptinotarsa decemlineata.

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