Femtosecond laser pulses distinguish bacteria from background urban aerosols

作者: François Courvoisier , Véronique Boutou , Vanessa Wood , Andreas Bartelt , Matthias Roth

DOI: 10.1063/1.2007870

关键词: LaserFemtosecondOptical pumpingBioluminescencePulse (signal processing)FluorescenceChemistryUltrashort pulseAnalytical chemistryAbsorption (electromagnetic radiation)

摘要: The fluorescence from living bacteria (Bacillus subtilis, Enteroccocus and Escherichia coli), induced by a ultrashort 270 nm pump pulse is depleted up to 50% an optically delayed ultrafast 810 probe in pump-probe arrangement. fast (subpicosecond) decrease occurs for delay of Δt>2ps. Depletion also observed tryptophan water contrast with organic cyclic molecules such as naphtalene or diesel fuel, despite similar absorption spectra. This remarkable difference allows us propose new remote sensing method able efficiently discriminate biological aerosols highly populated urban areas.

参考文章(22)
T. Brixner, N. H. Damrauer, P. Niklaus, G. Gerber, Photoselective adaptive femtosecond quantum control in the liquid phase Nature. ,vol. 414, pp. 57- 60 ,(2001) , 10.1038/35102037
Eva Leotz-Gartziandia, Véronique Tatry, Patrick Carlier, Sampling and Analysis of Organic Compounds in Diesel Particulate Matter Environmental Monitoring and Assessment. ,vol. 65, pp. 155- 163 ,(2000) , 10.1023/A:1006430107497
G. M�jean, J. Kasparian, J. Yu, S. Frey, E. Salmon, J.-P. Wolf, Remote detection and identification of biological aerosols using a femtosecond terawatt lidar system Applied Physics B. ,vol. 78, pp. 535- 537 ,(2004) , 10.1007/S00340-004-1466-X
Adam Kristensson, Christer Johansson, Roger Westerholm, Erik Swietlicki, Lars Gidhagen, Ulla Wideqvist, Vaclav Vesely, Real-world traffic emission factors of gases and particles measured in a road tunnel in Stockholm, Sweden Atmospheric Environment. ,vol. 38, pp. 657- 673 ,(2004) , 10.1016/J.ATMOSENV.2003.10.030
H. B. Steen, Wavelength dependence of the quantum yield of fluorescence and photoionization of indoles Journal of Chemical Physics. ,vol. 61, pp. 3997- 4002 ,(1974) , 10.1063/1.1681692
F. L. Reyes, T. H. Jeys, N. R. Newbury, C. A. Primmerman, G. S. Rowe, A. Sanchez, Bio-aerosol fluorescence sensor Field Analytical Chemistry and Technology. ,vol. 3, pp. 240- 248 ,(1999) , 10.1002/(SICI)1520-6521(1999)3:4/5<240::AID-FACT3>3.0.CO;2-#
Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Yong-Le Pan, Stephen Holler, Richard K. Chang, Jerold Bottiger, Bean T. Chen, Chun-Sing Orr, Greg Feather, Real-time measurement of fluorescence spectra from single airborne biological particles Field Analytical Chemistry and Technology. ,vol. 3, pp. 221- 239 ,(1999) , 10.1002/(SICI)1520-6521(1999)3:4/5<221::AID-FACT2>3.0.CO;2-7
J. D. Eversole, W. K. Cary, C. S. Scotto, R. Pierson, M. Spence, A. J. Campillo, Continuous Bioaerosol Monitoring Using UV Excitation Fluorescence: Outdoor Test Results Field Analytical Chemistry and Technology. ,vol. 5, pp. 205- 212 ,(2001) , 10.1002/FACT.1022
Ronald G. Pinnick, Steven C. Hill, Yong-Le Pan, Richard K. Chang, Fluorescence spectra of atmospheric aerosol at Adelphi, Maryland, USA: measurement and classification of single particles containing organic carbon Atmospheric Environment. ,vol. 38, pp. 1657- 1672 ,(2004) , 10.1016/J.ATMOSENV.2003.11.017
M. S. Zubairy, M. O. Scully, G. W. Kattawar, R. P. Lucht, T. Opatrny, H. Pilloff, A. Rebane, A. V. Sokolov, FAST CARS: Engineering a laser spectroscopic technique for rapid identification of bacterial spores Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 10994- 11001 ,(2002) , 10.1073/PNAS.172290899