MiR-23b/-27b Cluster Suppresses the Metastatic Phenotype of Aggressive Prostate Cancer Cells

摘要: of a dissertation at the University Miami. Dissertation supervised by Professor Kerry L. Burnstein. No. pages in text. (93) MicroRNAs (miRs) are small, endogenous, non-coding RNAs that regulate gene expressing inhibiting stability and/or translation complementary mRNA targets. Expression miR-23b/-27b, which encoded tandem as cluster on chromosome 9, specifically downregulated metastatic prostate cancer tissue samples compared to benign prostate. MiR-23b/-27b levels also lower aggressive cell lines more indolent cells. We show miR-23b/-27b suppress migration and invasion two human lines. Furthermore, inhibition increases potential less line. Interestingly, we found does not affect proliferation suggesting suppressors. provide evidence additional key tumorigenic processes. To understand mechanism mediated suppression processes, examined effects Rac1, Rho GTPase essential for phenotype ectopic expression decrease Rac1 activity while these miRs increase In addition, adhesion protein E-cadherin, independent Conversely line decreases E-cadherin levels. These data establish an important role mediatedsuppression Since have great use biomarkers drug targets or mimics, elucidating is critical development novel effective therapies prognostic indicators.