Overexpression of RIP140 suppresses the malignant potential of hepatocellular carcinoma by inhibiting NF‑κB‑mediated alternative polarization of macrophages.

摘要: Abstract Tumor-associated macrophages (TAMs) and their alternative activation contribute greatly to the development of hepatocellular carcinoma (HCC). Receptor-interacting protein 140 (RIP140) is widely expressed in regulates macrophage-mediated energy metabolism, inflammatory response tumorigenesis. However, whether RIP140 involved TAMs has not been reported. In present study, we determined expression after treatment with HCC-conditioned medium (HCM) for 24 h. We also analyzed effects overexpression on macrophage polarization, invasion apoptosis HepG2 Huh7 cells. Transwell assays were used estimated cell apoptosis. addition, investigated growth H22 cells by subcutaneous injection along BALB/c nude mice. Western blotting qRT-PCR detect protein mRNA associated NF-κB/IL-6 axis TAMs. Immunohistochemical immunofluorescence staining evaluate or F4/80 human HCC samples. The peripheral blood mononuclear was detected western blotting. Kaplan‑Meier survival curve estimation overall patients based found that HCM inhibited fostered macrophages. significantly inhibiting TAMs, suppressed both in vitro in vivo. monocytes lower than healthy people, this result consistent Furthermore, low high be closely correlated shorter time HCC.