Plastin3 is a novel marker for circulating tumor cells undergoing the epithelial-mesenchymal transition and is associated with colorectal cancer prognosis

摘要: Circulating tumor cells (CTC) in blood have attracted attention both as potential seeds for metastasis and biomarkers. However, most CTC detection systems might miss epithelial-mesenchymal transition (EMT)-induced metastatic because is based on epithelial markers. First, to discover novel markers capable of detecting CTCs which EMT has not been repressed, microarray analysis 132 colorectal cancers (CRC) from Japanese patients was conducted, 2,969 genes were detected that overexpressed relative normal colon mucosa. From the genes, we selected those CRC with distant metastasis. Then, analyzed metastasis-specific (n = 22) determine whether they expressed circulation. As a result, PLS3 discovered marker but Using fluorescent immunocytochemistry, validated EMT-induced peripheral PLS3-expressing approximately one-third an independent set 711 CRC. Multivariate showed PLS3-positive independently associated prognosis training 381) validation [n 330; HR 2.17; 95% confidence interval (CI) 1.38-3.40 3.92; CI 2.27-6.85]. The association between particularly strong Dukes B (HR 4.07; 1.50-11.57) C 2.57; 1.42-4.63). cells, it possesses significant prognostic value.