Ubiquitination of Cdc20 by the APC Occurs through an Intramolecular Mechanism

摘要: Summary Background Cells control progression through late mitosis by regulating Cdc20 and Cdh1, the two mitotic activators of anaphase-promoting complex (APC). The protein levels during cell cycle is not well understood. Results Here, we demonstrate that degraded in budding yeast multiple APC-dependent mechanisms. We find majority turnover does involve a second activator molecule but instead depends on cis autoubiquitination while it bound to its activator-binding site APC core. Unlike trans ubiquitination substrates, ubiquitinates independent activation Cdc20's C box. this intramolecular mechanism regulated, contributing decline occurs after anaphase. Interestingly, high substrate in vitro significantly reduce autoubiquitination. Conclusion show here fluctuates via a distinct form APC-mediated ubiquitination. This may preferentially occur early anaphase, following depletion substrates. suggests mechanisms are able target for at different points cycle.