Dehydroepiandrosterone inhibits lipopolysaccharide-induced nitric oxide production in BV-2 microglia.
作者： Mei-Jen Wang , Hsueh-Meei Huang , Huan-Lian Chen , Jon-Son Kuo , Kee-Ching G. Jeng
DOI: 10.1046/J.1471-4159.2001.00295.X
关键词: Internal medicine 、 Endocrinology 、 Nitric oxide synthase 、 Neuroprotection 、 Microglia 、 Cell culture 、 Dehydroepiandrosterone 、 Nitric oxide 、 Lipopolysaccharide 、 Neuroglia 、 Chemistry
摘要: Levels of dehydroepiandrosterone (DHEA) and its sulfated derivative (DHEAS) decline during aging reach even lower levels in Alzheimer's disease (AD). DHEA is known to exhibit a variety functional activities the CNS, including an increase memory learning, neurotrophic neuroprotective effects, reduction risk age-related neurodegenerative disorders. However, influence on immune functions glial cells poorly understood. In this study, we investigated effect activated glia. The production inducible nitric oxide synthase (iNOS) was studied lipopolysaccharide (LPS)-stimulated BV-2 microglia, as model activation. results showed that but not DHEAS significantly inhibited nitrite LPS-stimulated cell cultures. Pretreatment with reduced LPS-induced iNOS mRNA protein dose-dependent manner. activity decreased by exposure 100 µm DHEA. Moreover, suppressed gene expression did require de novo synthesis new proteins or destabilize mRNA. Since biosynthesized astrocytes neurons, our findings suggest it might have important regulatory function microglia.
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