Arginine as an effective additive in gel permeation chromatography
作者： Daisuke Ejima , Ryosuke Yumioka , Tsutomu Arakawa , Kouhei Tsumoto
DOI: 10.1016/J.CHROMA.2005.07.086
关键词: Gel permeation chromatography 、 Phase (matter) 、 Chemistry 、 Basic fibroblast growth factor 、 Monoclonal antibody 、 Chromatography 、 Size-exclusion chromatography 、 Aqueous solution 、 Arginine 、 Recombinant DNA 、 Organic chemistry 、 Analytical chemistry 、 Biochemistry 、 General Medicine
摘要: A major problem in gel permeation chromatography (GPC) or size exclusion is non-specific binding of applied proteins to the column matrix (stationary phase). We have tested an aqueous arginine solution as GPC mobile phase on silica-based and polymer-based columns, using mouse monoclonal antibody recombinant human activin, interleukin-6, basic fibroblast growth factor, interferon-γ model proteins. observed that addition improves separation their soluble aggregates from which suggests effective additive for phase.
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