Multidrug resistance characterization in multicellular tumour spheroids from two human lung cancer cell lines

摘要: Most of the knowledge about mechanisms multidrug resistance in lung cancer has been achieved through use cell lines isolated from tumours cultivated either suspensions cells or monolayers and following exposition to different cytostatic agents. However, tumour growing as multicellular spheroids (MTS) frequently develop a drug-independent form. The aim this study was characterize phenotypic functional differences between two human NSCLC (INER-37 INER-51) grown traditional monolayer cultures versus MTS. After 72 hours treatment with anticancer drugs, chemosensitivity assessed using MTT assay. Reverse transcription-polymerase chain reaction employed detect mRNAs resistance-related genes. expression P-gp analyzed by immunohistochemical staining cycle profiles were FACS. results indicate that when MTS each line had morphologies well abrogation proliferation decrease G2/M phase. Also, acquired several chemotherapeutic agents only few days culture which accomplished significant changes MDR-related Overall, changed cellular response drugs nevertheless studied seems implement acquire resistance.