The differentiation pathway of HL60 cells is a model system for studying the specific regulation of some myeloid genes.

摘要: During granulopoiesis, certain myeloid genes encoding products of azurophilic granules are specifically down-regulated. The specific enzyme myeloperoxidase belongs to this group genes. It is responsible for the production hypochlorous acid, a potent microbicidal agent which involved in host defense. induced differentiation promyelocytic leukemic HL60 cells granulocyte- or monocyte-like cells, RNA depressed. We studied depression process more detail by limiting exposure inducer phorbol 12-myristate 13-acetate 24 h. time period, no significant decrease cell number and viability could be observed. Analysis these vitro differentiated on protein levels showed that they can used under defined conditions as system study Under described conditions, both transcriptional rate gene well stability its transcript was reduced.