Utilization and cooxidation of chlorinated phenols byPseudomonas sp. B 13

摘要: Pseudomonas sp. B13 was grown in continuous culture on 4-chlorophenol as the only carbon source. Maximum growth rate of 0.4h-1 observed at a substrate concentration >0.01 mM and <0.15 mM. In addition to enzymes phenol catabolism, high specific 1,2-dioxygenase activities with chlorocatechols substrates were found. The isomeric monochlorinated phenols also totally degraded by cells. (+)-2,5-Dihydro-4-methyl- (+)-2,5-dihydro-2-methyl-5-oxo-furan-2-acetic acid formed yield dead-end catabolites from cooxidation cresoles. Several dichlorophenols except 2,6-dichlorophenol removed fluid chlorophenol Ring cleavage chlorinated catechols shown be one critical steps catabolism. A catabolic pathway for chlorophenols is discussed.