Ultrastructural and physiological examination of oospore dormancy and germination in Pythium aphanidermatum

摘要: Developmental events in the germination of oospores Pythium aphanidermatum were studied with electron microscope. Culturally grown oospores, fed to aquatic snails (Planorbis sp.), germinated maximally within a 12-hour period. Mature, dormant, ingested maintained distilled water and various stages liquid nutrient broth on corn meal agar examined. A single central globule, 6 11 pm diameter, numerous storage bodies, 0.4 1.7 comprised endogenous re­ serves dormant oospore. During germination, globule broke up into several smaller bodies underwent shrinkage as it was absorbed adjacent vacuoles. Utilization peripheral cytoplasm also appeared be associated The oospore wall considerable morphological changes during germina­ tion, based light microscopic observations, further aspect study dealt certain physiological properties non­ snail-ingested order compare adenosine triphosphate levels, oxygen consumption, permeability