Extraction of cephalosporin C from whole broth and separation of desacetyl cephalosporin C by aqueous two‐phase partition
作者： Wu-Yung Yang , Chun-Der Lin , I-Ming Chu , Chau-Jen Lee
关键词: Ammonium sulfate 、 PEG ratio 、 Partition coefficient 、 Aqueous solution 、 Salt (chemistry) 、 Cephalosporin C 、 Chromatography 、 Chemistry 、 Extraction (chemistry) 、 Acetone
摘要: Cephalosporin C was extracted from diluted or whole broth by PEG/salt aqueous two-phase systems. Parameters such as PEG molecular weight, salt type, pH, and concentration were investigated for finding a suitable extraction system. In 600/ammonium sulfate phosphate systems, K(c) (partition coefficienct of cephalosporin C) observed to be larger than 1, with K(d) coefficient desacetyl being smaller 1. The particular values these coefficients would imply that the difficult separation could possibly achieved via extraction. addition surfactants, water-miscible solvents, neutral salts enhancement efficiency also investigated. surfactants system did not affect substantially. increase whereas decreased result acetone, MeOH, EtOH, IPA, n-BuOH. Meanwhile both decrease whenever salts, NaCl, KCl, Kl, KSCN, added. partitioning behavior in filtered, whole, different batches notably quite similar broth. recovery yield function centrifugal force used phase separation. (c) 1994 John Wiley & Sons, Inc.
sci-hub.se 参考文章(28)
David F Ollis, James Edwin Bailey, Biochemical engineering fundamentals Published in <b>1986</b> in New York by McGraw-Hill. ,(1986)
Per-Åke Albertsson, Bertil Andersson, Separation of membrane components by partition in detergent-containing polymer phase systems Journal of Chromatography A. ,vol. 215, pp. 131- 141 ,(1981) , 10.1016/S0021-9673(00)81394-1
Mark A. Eiteman, John L. Gainer, The effect of free-volume changes on partitioning in magnesium sulfate-poly(ethylene glycol) aqueous two-phase systems Biochimica et Biophysica Acta. ,vol. 992, pp. 125- 127 ,(1989) , 10.1016/0304-4165(89)90058-5
Rajiv Datar, Carl-Gustaf Rosén, Studies on the removal of Escherichia coli cell debris by aqueous two-phase polymer extraction Journal of Biotechnology. ,vol. 3, pp. 207- 219 ,(1986) , 10.1016/0168-1656(86)90003-9
Andres Veide, Torgny Lindbäck, Sven-Olof Enfors, Continuous extraction of β-d-galactosidase from Escherichia coli in an aqueous two-phase system: effects of biomass concentration on partitioning and mass transfer Enzyme and Microbial Technology. ,vol. 6, pp. 325- 330 ,(1984) , 10.1016/0141-0229(84)90062-0
Cheng-Kang Lee, Stanley I. Sandler, Vancomycin partitioning in aqueous two-phase systems: effects of pH, salts, and an affinity ligand. Biotechnology and Bioengineering. ,vol. 35, pp. 408- 416 ,(1990) , 10.1002/BIT.260350408
Jonathan Huddleston, Andres Veide, Kristina Köhler, Julia Flanagan, Sven-Olof Enfors, Andrew Lyddiatt, The molecular basis of partitioning in aqueous two-phase systems Trends in Biotechnology. ,vol. 9, pp. 381- 388 ,(1991) , 10.1016/0167-7799(91)90130-A
Göte Johansson, Gerhard Kopperschläger, Effects of organic solvents on the partitioning of enzymes in aqueous two-phase systems Journal of Chromatography A. ,vol. 388, pp. 295- 305 ,(1987) , 10.1016/S0021-9673(01)94491-7
I-Ming Chu, Shiao-Li Chang, Shi-Huang Wang, Wu-Yung Yang, Extraction of amino acids by aqueous two-phase partition Biotechnology Techniques. ,vol. 4, pp. 143- 146 ,(1990) , 10.1007/BF00163289
Boris Y. Zaslavsky, Anna A. Borvskaya, Nelli D. Gulaeva, Larisa M. Miheeva, Physico-chemical features of solvent media in the phases of aqueous polymer two-phase systems. Biotechnology and Bioengineering. ,vol. 40, pp. 1- 7 ,(1992) , 10.1002/BIT.260400102