Sites of serotonin uptake in epithelia of the developing mouse palate, oral cavity, and face: possible role in morphogenesis.

摘要: With the method of whole mouse embryo culture, together with immunocytochemistry an antiserum to serotonin (5-HT), sites 5-HT uptake were found be transiently expressed in epithelia developing palate, tongue, nasal septum, and maxillary mandibular prominences during period active morphogenesis (embryonic days 12-14; or E12-14). These had ability take up when added culture medium presence MAO inhibitor nialamide antioxiant, L-cysteine (NC), could also seen after exposure embryos precursor L-tryptophan (L-TRP) + NC. visible culturing without any additives, which may have been due L-TRP one component (DMEM) itself, is present relatively high amounts fetal calf serum. At E12-13, appearance immunoreactivity (IR) at these treatment NC was blocked by fluoxetine, providing further evidence that are true uptake. However, fluoxetine did not completely block E14 although it effective alone. This finding mean into occurs mechanisms there some limited capacity for synthesis. Taken results from previous studies where 1) has reported stimulate palatal shelf reorientation mesenchyme cell motility vitro [Wee et al., J Embryol Exp Morphol 53:75-90, 1979; Zimmerman Craniofac Genet Dev Biol 3:371-385, 1983] 2) long-term shown cause malformations craniofacial region (Lauder, Thomas, Sadler, preparation), study suggest act as a developmental signal oral cavity, face morphogenesis.