作者: David J. McConkey , Curtis A. Pettaway , Graham Greene
DOI:
关键词: Carcinoma 、 LNCaP 、 Apoptosis 、 Prostate 、 Metastasis 、 DNA fragmentation 、 Endocrinology 、 Biology 、 Cancer research 、 Programmed cell death 、 Prostate cancer 、 Internal medicine
摘要: Abstract The aim of this study was to determine whether stable differences in apoptosis sensitivity were selected for nonmetastatic and metastatic variants the LNCaP human prostate carcinoma line that had been isolated from tumors grown orthotopically glands regional lymph nodes nude mice. LNCaP-Pro5 cells significantly more sensitive thapsigargin-induced than LNCaP-LN3 cells, as measured by viability, DNA fragmentation, interleukin 1β-converting enzyme family-mediated cleavage repair enzyme, poly(ADP-ribose) polymerase. Apoptosis resistance associated with higher levels expression cell death suppressor BCL-2 lower promoters BAX BAK detected whereas two other family members (BCL-XL BAD) indistinguishable. Our data support hypothesis contributes cancer metastasis elevated is involved.