Apoptosis Resistance Increases with Metastatic Potential in Cells of the Human LNCaP Prostate Carcinoma Line

作者: David J. McConkey , Curtis A. Pettaway , Graham Greene

DOI:

关键词: CarcinomaLNCaPApoptosisProstateMetastasisDNA fragmentationEndocrinologyBiologyCancer researchProgrammed cell deathProstate cancerInternal medicine

摘要: Abstract The aim of this study was to determine whether stable differences in apoptosis sensitivity were selected for nonmetastatic and metastatic variants the LNCaP human prostate carcinoma line that had been isolated from tumors grown orthotopically glands regional lymph nodes nude mice. LNCaP-Pro5 cells significantly more sensitive thapsigargin-induced than LNCaP-LN3 cells, as measured by viability, DNA fragmentation, interleukin 1β-converting enzyme family-mediated cleavage repair enzyme, poly(ADP-ribose) polymerase. Apoptosis resistance associated with higher levels expression cell death suppressor BCL-2 lower promoters BAX BAK detected whereas two other family members (BCL-XL BAD) indistinguishable. Our data support hypothesis contributes cancer metastasis elevated is involved.

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