The chain weight of wool keratin.

作者: W.R. Middlebrook

DOI: 10.1016/0006-3002(51)90068-6

关键词: SerineSilica gelGlycineAlanineAspartic acidThreonineHydrolysisChemistryChromatographyValine

摘要: 1. 1. A method is described for the separation of DNP-amino-acids, using buffered silica gel columns. 2. 2. By treatment with FDNB, and DNP-amino-acids after hydrolysis, wool shown to contain seven different amino-acid residues having free α-amino groups i.e. valine, alanine, glycine, threonine, serine, glutamic acid aspartic acid. 3. 3. The end-groups have been quantitatively estimated average chain weight determined. 4. 4. molecular model proposed which in accordance these values.

参考文章(30)
R. R. Porter, F. Sanger, The free amino groups of haemoglobins. Biochemical Journal. ,vol. 42, pp. 287- 294 ,(1948) , 10.1042/BJ0420287
Kenneth Bailey, The denaturation of edestin by acid: T. B. Osborne's edestan Biochemical Journal. ,vol. 36, pp. 140- 154 ,(1942) , 10.1042/BJ0360140
A. H. Gordon, A. J. P. Martin, R. L. M. Synge, Partition chromatography in the study of protein constituents. Biochemical Journal. ,vol. 37, pp. 79- 86 ,(1943) , 10.1042/BJ0370079
F. Sanger, The terminal peptides of insulin Biochemical Journal. ,vol. 45, pp. 563- 574 ,(1949) , 10.1042/BJ0450563
A. C. Chibnall, M. W. Rees, E. F. Williams, The total nitrogen content of egg albumin and other proteins. Biochemical Journal. ,vol. 37, pp. 354- 359 ,(1943) , 10.1042/BJ0370354
F. Sanger, The free amino groups of insulin. Biochemical Journal. ,vol. 39, pp. 507- 515 ,(1945) , 10.1042/BJ0390507
R. S. DE ROPP, Action of streptomycin on plant tumours. Nature. ,vol. 162, pp. 459- 460 ,(1948) , 10.1038/162459A0
A. J. P. Martin, R. L. M. Synge, A new form of chromatogram employing two liquid phases Biochemical Journal. ,vol. 35, pp. 1358- 1368 ,(1941) , 10.1042/BJ0351358