作者: W.R. Middlebrook
DOI: 10.1016/0006-3002(51)90068-6
关键词: Serine 、 Silica gel 、 Glycine 、 Alanine 、 Aspartic acid 、 Threonine 、 Hydrolysis 、 Chemistry 、 Chromatography 、 Valine
摘要: 1. 1. A method is described for the separation of DNP-amino-acids, using buffered silica gel columns. 2. 2. By treatment with FDNB, and DNP-amino-acids after hydrolysis, wool shown to contain seven different amino-acid residues having free α-amino groups i.e. valine, alanine, glycine, threonine, serine, glutamic acid aspartic acid. 3. 3. The end-groups have been quantitatively estimated average chain weight determined. 4. 4. molecular model proposed which in accordance these values.