Pregnancy-associated glycoprotein (PAG) family localized in chorionic cells within the epitheliochorial/diffuse placenta of the alpaca (Lama pacos).
作者： Marta Majewska , Grzegorz Panasiewicz , Bozena Szafranska
DOI: 10.1016/J.ACTHIS.2010.06.002
关键词: Placentation 、 Internal medicine 、 Immunohistochemistry 、 Days post coitum 、 Endocrinology 、 Propidium iodide 、 Placenta 、 Biology 、 Pregnancy 、 Andrology 、 Trophoblast 、 Glycoprotein
摘要: Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and later post-implantational chorionic epithelium of some ungulate species. This paper describes cellular immunolocalization PAG family epitheliochorial placenta type alpaca (Lama pacos-Lp), which PAGs were named 'LpPAGs'. Placental Lp sections (5 μm) different females near mid-pregnancy (150 days post coitum; dpc), advanced pregnancy (244-263 dpc) late (347 used for cross-species (heterologous-ht) double fluorescent immunohistochemistry (htdF-IHC). The htdF-IHC was performed with primary rabbit polyvalent anti-porcine polyclonals. LpPAG immuno-complexes visualized secondary goat anti-rabbit immunoglobulins-conjugated Alexa 488 fluorophore (green), among all nuclei placental cells stained propidium iodide (red). is first study reporting identified by at feto/maternal interface during stages alpaca. most dominant strongest immune-positive signals found well-developed cell layer. Our indicated relatively high epitope resemblance to that camelids pigs. These data increase our general knowledge localization pregnancy-stage dependent development diffuse
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