Immune Profiling Panel: a proof of concept study of a new multiplex molecular tool to assess the immune status of critically-ill patients

摘要: Abstract Background Critical illness such as sepsis is a life-threatening syndrome defined dysregulated host response to infection and characterized by patients exhibiting various impaired immune profiles. In the field of diagnosis, gap still remains in identifying profile critically-ill ICU. The availability an profiling tool holds great potential providing at high risk with more accurate precise management. this study, multiplex panel prototype was assessed for its ability semi-quantify markers directly from blood, using FilmArray® System. Results Immune Profiling Panel (IPP) consists 16 biomarkers that target both innate adaptive responses, pro- anti-inflammatory mediators well genes involved diverse regulatory pathways. analytical studies carried out on healthy volunteers showed minimal inter- intra-variability testing samples across tested lots. majority assays were linear R2 higher than 0.8. IPP pouch comparable qPCR within limits agreement. Finally, quantification cycle values normalized against reference account different composition cells among specimens. use selected demonstrated gene modulations could distinctly differentiate three profiles: healthy, borderline mHLA-DR septic shock low patients. Conclusion allowed transcriptomic analysis whole blood less hour. development fully automated, rapid easy-to-use tool, enabling stratification