Customized Scaffold Design Based on Natural Peripheral Nerve Fascicle Characteristics for Biofabrication in Tissue Regeneration

摘要: Objective. The use of a biofabrication nerve scaffold, which mimics the microstructure, as an alternative for autologous transplantation is promising strategy treating peripheral defects. This study aimed to design customized scaffold model with characteristics human fascicles. Methods. We used Micro-MRI technique obtain different A full-length 28 cm tibial specimen was obtained and divided into 14 two-centimetre segments. 3D models fascicles were by three-dimensional reconstruction after image segmentation. central line fitted, aggregation analysed quantitatively. designed simulating clinical defect extracting information from acquired fascicle data; displayed printing verify accuracy model. Result. microstructure sciatic nerve, common peroneal in could be reconstruction. number cross fusions proximal end distal decreased gradually. By designing graft accordance fascicles, printed demonstrated that two ends can well matched. Conclusion. complicated changeable, spatial position each long segment show great changes at levels. Under premise stability existing imaging techniques, large scanning samples set up database integrating gross information, provide template downstream