Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

摘要: ABSTRACT Microbial fermentation of agro-industrial waste holds great potential for reducing the environmental impact associated with production lipids industrial purposes from plant biomass. However, chemical complexity many residues currently prevents efficient conversion into lipids, creating a high demand strains ability to utilize all energy-rich components agricultural residues. Here, we present results genome and transcriptome analyses Trichosporon oleaginosus . This oil-accumulating yeast is able grow on wide variety substrates, including pentoses N -acetylglucosamine, making it an interesting candidate biotechnological applications. Transcriptomics shows specific changes in gene expression patterns under lipid-accumulating conditions. Furthermore, content indicate that T. oleaginosus well-adapted utilization chitin-rich We also focused mating type, because this species member Tremellomycetes , group has been intensively analyzed as model evolution sexual development, best-studied being Cryptococcus neoformans. The structure mating-type regions differs significantly other reveals new evolutionary trajectory paradigm. Comparative analysis recruitment developmental genes ancestral tetrapolar loci occurred independently lineages, supporting hypothesis trend toward larger fungi. IMPORTANCE Finite fossil fuel resources pose sustainability challenges society industry. oils are sustainable feedstock biofuel does not compete food production. describe oleaginous which can accumulate up 70% its dry weight lipids. In contrast conventional yeasts, organism only absence diauxic effect while fermenting hexoses but effectively utilizes xylose building blocks lignocellulose chitin, respectively. Transcriptome revealed metabolic networks govern or -acetylglucosamine well lipid accumulation. These data form basis targeted strain optimization strategy. type supports fungi, similar sex chromosomes animals plants.